Chinese Journal of Plastic and Reconstructive Surgery

2021, v.3(02) 63-67+75

[打印本页] [关闭]
本期目录(Current Issue) | 过刊浏览(Past Issue) | 高级检索(Advanced Search)

PD-L1 Expression and Tumor Infiltrating Lymphocytes in Neurofibromatosis Type 1-Related Benign Tumors and Malignant Peripheral Nerve Sheath Tumors: An Implication for Immune Checkpoint Inhibition Therapy
PD-L1 Expression and Tumor Infiltrating Lymphocytes in Neurofibromatosis Type 1-Related Benign Tumors and Malignant Peripheral Nerve Sheath Tumors: An Implication for Immune Checkpoint Inhibition Therapy

Jin LIU;Haibo LI;Chengjiang WEI;Qingfeng LI;Zhichao WANG;

摘要(Abstract):

Background Neurofibromatosis type 1(NF1) is an autosomal dominant inherited disorder. It can affect multiple systems of the body and cause severe disfigurement and discomfort in these patients. There are two types of neurofibromas, named cutaneous and plexiform neurofibromas. The latter type may transform into malignant peripheral nerve sheath tumors(MPNSTs). Surgical resection is difficult to perform owing to the complex tissue structure of neurofibromas; therefore, it is necessary to develop novel and effective therapies for the treatment of these tumors. Programmed cell death protein 1(PD-1)/programmed cell death-ligand 1(PD-L1)-related immune checkpoint inhibitors have been proven effective for various cancers, and the positive expression of PD-L1 and tumor-infiltrating lymphocytes(TILs) has been recognized as a biomarker for the response to immune checkpoint therapy.Methods We conducted immunohistochemistry(IHC) staining to detect PD-L1 expression in plexiform neurofibroma and MPNST tissue samples. Reverse transcription-polymerase chain reaction(RT-PCR) and western blotting were performed to detect PD-L1 and PD-1 expression in MPNST cell lines. IHC staining was used to show immune cell infiltration in NF1 and MPNST tissues. Results IHC staining showed PD-L1 positive expression in neurofibromas and MPNST tumor tissues. In addition, q PCR and western blotting showed high expression of PD-L1 in MPNST tumor cells. IHC staining revealed that aberrant T lymphocytes infiltrated the plexiform neurofibroma and MPNST tumor tissues.Conclusion These results indicate that immune checkpoint mechanisms may play a pivotal role in the development of NF1-related tumors, and immune checkpoint inhibitors may be effective for managing neurofibromas and MPNSTs. Combined therapy with other molecular agents may be explored in the future.

关键词(KeyWords):

Abstract:

Keywords:

基金项目(Foundation): supported by the grants from the Youth Doctor Collaborative Innovation Team Project (QC201803) of Shanghai Ninth People’s Hospital of Shanghai Jiao Tong University School of Medicine,Shanghai Youth Top-Notch Talent Program (201809004);; “Chenguang Program” supported by Shanghai Education Development Foundation and Shanghai Municipal Education Commission (19CG18);; Science and Technology Commission of Shanghai Municipality (19JC1413);; Shanghai Rising Star Program (20QA1405600);; Innovative research team of high-level local universities in Shanghai (SSMUZDCX20180700);; Shanghai Municipal Key Clinical Specialty (shslczdzk00901)

作者(Author): Jin LIU;Haibo LI;Chengjiang WEI;Qingfeng LI;Zhichao WANG;

Email:

DOI:

参考文献(References):

扩展功能
本文信息
服务与反馈
本文关键词相关文章
本文作者相关文章
中国知网
分享